Figure 1.

T_b and Body Weight Are Progressively Reduced during CR until Reaching a Plateau

  • Figure 1A

    (A) Tb profile of C57BL/6 male mice from AL to 50% CR. The progressive reduction in Tb was analyzed by one-way ANOVA, R2 = 0.3637, F(20, 967) = 27.64, p <0.0001, followed by posttest for linear trend, slope = 0.24, p < 0.0001.

    n = 6 mice; graphs show mean ± SEM. For the experimental design, see Figure S1.

  • Figure 1B

    B) Description goes h(B) Detailed 24-h Tb and locomotor activity profiles during AL and 50% CR at day 13; the arrow indicates the time at which food was given during CR.

    n = 6 mice; graphs show mean ± SEM. For the experimental design, see Figure S1.

  • Figure 1C

    (C and D) Daily (C) and cumulative (D) body weight loss percentages of C57BL/6 male mice subjected to CR.

    (C) Repeated-measures (RM) one-way ANOVA between days of the CR regimen, F(4.486, 22.43) = 16.53, p < 0.0001, followed by Tukey’s multiple-comparisons test, p < 0.05.

    n = 6 mice; graphs show mean ± SEM. For the experimental design, see Figure S1.

  • Figure 1D

    (C and D) Daily (C) and cumulative (D) body weight loss percentages of C57BL/6 male mice subjected to CR.

    (D) The reduction in body weight over time was analyzed by RM one-way ANOVA, R2 = 0.7606, F(1, 260) = 6127, p < 0.0001, followed by posttest for linear trend, slope = 0.49, p < 0.001.

    n = 6 mice; graphs show mean ± SEM. For the experimental design, see Figure S1.